Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus.
نویسندگان
چکیده
منابع مشابه
Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus.
This study followed the transmission of 64 segregating genetic markers to 52 haploid offspring, obtained from both homokaryotic and heterokaryotic meiospores, of a cross (AG 93b) of Agaricus bisporus, the commonly cultivated "button mushroom." The electrophoretic karyotypes of the AG 93b component nuclei were determined concurrently (n = 13). Eleven distinct linkage groups were identified by tw...
متن کاملPersistent meiotic arrest in basidia of Agaricus bisporus
The pattern of distribution of genetic markers among progeny of Agaricw bisporus, whether conventional mutants, electrophoretic isozyme variants or restriction fragment length polymorphisms, implies a combination of reduced recombination rates and preferential segregation of centromeres during meiosis. We have considered an alternative explanation, namely that meiosis may be an infrequent event...
متن کاملA meiotic DNA polymerase from a mushroom, Agaricus bisporus.
A meiotic DNA polymerase [DNA nucleotidyltransferase (DNA-directed), EC 2.7.7.7], which likely has a role in meiotic DNA repair, was isolated from a mushroom, Agaricus bisporus. The purified fraction displays three bands in SDS/PAGE, at molecular masses of 72 kDa, 65 kDa and 36 kDa. Optimal activity is at pH 7.0-8.0 in the presence of 5 mM Mg2+ and 50 mM KCl and at 28-30 degrees C, which is the...
متن کاملThe Genetic Linkage Map of the Medicinal Mushroom Agaricus subrufescens Reveals Highly Conserved Macrosynteny with the Congeneric Species Agaricus bisporus
Comparative linkage mapping can rapidly facilitate the transfer of genetic information from model species to orphan species. This macrosynteny analysis approach has been extensively used in plant species, but few example are available in fungi, and even fewer in mushroom crop species. Among the latter, the Agaricus genus comprises the most cultivable or potentially cultivable species. Agaricus ...
متن کاملAgrobacterium tumefaciens mediated transformation of Agaricus bisporus
Agrobacterium tumefaciens is known to transfer parts of its tumor-inducing plasmid to filamentous fungi including Agaricus bisporus. We have used this transformation system to transform germinating basidiospores of A. bisporus. Analysis of hygromycin B-resistant clones showed that the T-DNA integrated at random sites and in multiple copies into the host genome. Furthermore we have been able to ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genetics
سال: 1993
ISSN: 1943-2631
DOI: 10.1093/genetics/133.2.225